Test of the Healing Effect of Ethanol Extract of African Leaves (Vernonia amygdalina Del.) on Cuts on Mice (Mus musculus)

Abstract

Natural medicine preparations as part of Indonesia's national cultural heritage are now increasingly important in everyday life. People have used various plants as traditional medicines. This study identified secondary metabolic compounds and tested the effectiveness of 96% ethanol extract of African leaves (Vernonia amygdalina Del.) in healing cuts in mice (Mus musculus). African leaves (Vernonia amygdalina Del.) are medicinal plants found in East Kalimantan. This experimental study used phytochemical screening and effectiveness test methods, as well as one-way ANOVA analysis. The test animals were mice (Mus musculus), which were divided into five groups. They consisted of a positive control group using 10% povidone iodine, a negative control group using distilled water, and a treatment group with extract concentrations of 0.1 grams, 0.2 grams, and 0.3 grams. The results showed that 96% ethanol extract of African leaves (Vernonia amygdalina Del.) contained alkaloids, flavonoids, saponins, and tannins. In addition, in the effectiveness test of 96% ethanol extract of African leaves (Vernonia amygdalina Del.) can reduce cuts in test animals mice (Mus musculus), with the results of the wound length test showing significant results from the one way ANOVA analysis (F>F crit = 151.549>1.961) which shows that the effect of 96% ethanol extract of African leaves is effective for healing cuts with the best extract concentration being 0.3 g extract.